WebOct 28, 2024 · 4T1 cells were seeded in six-well plates with a coverslip in each well and incubated with DiD+FAM-CpG-S or DiD+FAM-CpG-N/A as above. At 2 or 4 h post-treatment, cells were fixed with 4% polyformaldehyde, washed twice with phosphate-buffered saline (PBS), and stained with DAPI (0.5 μg/mL; Biofroxx, Einhausen, … http://www.saiguobio.com/brand.aspx?id=61
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WebFeb 16, 2024 · Pulmonary fibrosis is a type of unrepairable and difficult-to-treat progressive interstitial lung disease, of which idiopathic pulmonary fibrosis (IPF) is the most severe [].IPF usually occurs in adults, causing dyspnoea and dry cough, and has a median survival of 2–3 years [2, 3].Pulmonary fibrosis is caused by ageing, genetic factors, environmental … WebAug 6, 2024 · Then, 20 μL of MTT (APExBIO, Houston, USA) was added to each well and incubated at 37°C in the dark for 4 h (16,17). After this, the plate was centrifuged at 4000 … WebMar 15, 2024 · E The MTT assay was used to test cell viability in N2A and HT22 ... The Lipofectamine 3000 reagent was obtained from Invitrogen (Waltham, MA, USA). The BSA was purchased from Biofroxx ... how can a cpa lose their license