Binary vector pcambia-1300
http://www.biovector.net/product/1319.html WebApr 7, 2024 · How to order pCAMBIA 1300 vector? Question. 3 answers. Apr 3, 2024; ... The steps include cloning your gene into a binary vector, transferred the vector into Agrobacterium, ...
Binary vector pcambia-1300
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WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a …
WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents WebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,...
WebJul 1, 2024 · The pCAMBIA 1300 plasmid containing a GFP reporter gene driven by the Super promoter and harboring a Hygromycin resistance gene was used for transformation of somatic embryos. ... we transfected embryogenic calli with A. tumefaciens strain GV3101 containing a pCAMBIA 1300 binary vector, ... WebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure …
WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a …
WebJun 1, 2007 · The cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants. greater full gospel church hurlockWebA. tumefaciens strains EHA 101 and EHA 105 contained the disarmed plasmid pCAMBIA 1304 which contains genes for hygromycin (hyg), β-gluconidase (gusA) and gfp (Fig. 2). The DHα E.coli strain ... flingpordnewsWebNov 9, 2015 · For genetic complementation, the full-length genomic DNA of the VPT1 fragment was amplified from wild type genomic DNA and cloned into the binary vector pCAMBIA-1300. To generate the VPT1VPT1 coding sequence without a stop codon, and GFP (35S:VPT1-GFP) were cloned into the pCAMBIA-1300. The tonoplast marker, … greater ft lauderdale food and wine festivalWebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β … greater full assurance ministries historyWebBinary vector pCAMBIA 1301 showing hptII gene near left border and GUS gene near right border of T-DNA from publication: An Efficient Protocol for the Agrobacterium-mediated Genetic Transformation ... greater ft myers chamber of commerceWebJul 24, 2013 · A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase ( hpt) marker gene. flingposse ロゴWebEnter the email address you signed up with and we'll email you a reset link. flingposse 壁紙